Abstract A modified serum-based bioassay was used to screen plant extracts and assess compound activity against newly hatched larvae of the Australian sheep blowfly Lucilia cuprina. Larval instar mortality and physical appearance were recorded after exposure for 24 h to the treatments. Extracts from New Zealand gymnosperms shown to have activity against L. cuprina larvae were: Podocarpus totara; P. acuteafolius; Dacrycarpus dacrydioides; Halocarpus bidwillii; H. biformis; H. kirkii; Lagarostrobos colensoi; Lepidothamnus intermedius; L. laxifolius; Phyllocladus trichomanoides; P. glaucus, and Agathis australis. The phytoecdysones 20-hydroxyecdysone, 5,20-dihydroxyecdysone, and ponasterone A inhibited larval moulting. Dyshomoerythrine, dacrysterone, and nagilactone C were insecticidal, with the latter also retarding growth at sublethal rates.
Keywords Lucilia cuprina; plant extracts; Gymnospermae; 20-hydroxyecdysone; 5,20-dihydroxyecdysone; ponasterone; nagilactone; dacrysterone; dyshomoerythrine
New Zealand Journal of Agricultural Research, 1997, Vol. 40: 261-267
0028-8233/97/4002-0261 $7.00/0 (c) The Royal Society of New Zealand 1997
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