Abstract Ryegrass seedlings with an unknown endophyte infection status were grown in hydroponic culture for 5 weeks as part of a ryegrass evaluation, to allow selections on different root types to be made. Of the 1920 plants grown, 132 were selected and planted out in the field. After a year, two ramets of each of the 132 plants were grown in hydroponic culture for a second period of 5 weeks and the level of endophyte infection was only then assessed. Endophyte strands were found in 98% of the 264 plants. Of each of the 5 endophyte-free plants, the duplicate plant contained endophyte. Endophyte infection was 100% in 18 of the 20 half-sibling (HS) ryegrass families used and 93% and 67% in the remaining two HS families. It is concluded that growing endophyte-infected perennial ryegrass plants in hydroponic culture has a minimal effect on the endophyte infection level.
Keywords hydroponic culture; perennial ryegrass; Lolium perenne; Neotyphodium lolii; endophyte
New Zealand Journal of Agricultural Research, 1999, Vol. 42: 513-514
0028-8233/99/4204-0513 $7.00/0 (c) The Royal Society of New Zealand 1999
Short communication
PDF file of entire paper: medium quality (193K); (scanned from paper original: notes about this process)