Abstract Interspecific hybridisation between tetraploid Lolium perenne L. (Lp) and L. multiflorum Lam. (Lm) was investigated by using morphological genetic markers. Fluorescent-root and awned-floret phenotypes are dominant in Italian ryegrass (L. multiflorum), so seed-parent Italian ryegrass plants were emasculated to eliminate the possibility of self-fertilisation. The percentages of germination were 84.3% in Lp × Lm and 90.6% in Lm × Lp crosses. The fluorescent seedlings were 81.8% in Lp × Lm and 83.7% in Lm × Lp crosses, and interspecific hybrids were 88.8 and 97.3%, respectively. The percentage of self-fertilisation was 4.0% in the perennial ryegrass. Pollen viability was 90.2% in the Lp × Lm and 91.0% in the reciprocal cross. The direction Lp × Lm seems to be a suitable method to produce interspecific hybrids on a mass scale.
Keywords awned floret; fluorescent root;
interspecific
hybridisation; L. perenne; L. multiflorum
A06041; Online publication date 2 July 2007; Received 30 March 2006;
accepted 21 March 2007
New Zealand Journal of Agricultural Research, 2007, Vol. 50:
279–284
0028–8233/07/5003–0279 © The Royal Society of New Zealand 2007
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