New Zealand Journal of Agricultural Research abstracts

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Application of comparative genomics in chicken gene mapping

Yong Tian^1,2

Lizhi Lu^2,*

Yan Fu¹

Deqian Wang²

Guoqin Li²

Aiping Yuan²

Qingyan Yuan²

Jin Zhao³

¹Laboratory of Animal Genetics, College of Animal Sciences
Zhejiang University
268 Kaixuan Road
Hangzhou 310029, PR China

²Institute of Animal Husbandry and Veterinary Science, Zhejiang Academy of Agricultural Sciences
198 Shiqiao Road
Hangzhou 310021, PR China

³College of Life Sciences, China Jiliang University
258 Xueyuan Road
Hangzhou 310018, PR China

^*Author for correspondence: lulizhibox@163.com

Abstract    The NDRG4 gene is a member of the N-myc downregulated gene family which belongs to the alpha/beta hydrolase superfamily. The protein encoded by this gene is a cytoplasmic protein that may be involved in the regulation of mitogenic signalling in vascular smooth muscle cells. To map this gene in the chicken chromosome, a 6000 rads chicken-hamster radiation hybrid panel (ChickRH6) was used. Primers were designed according to the published human sequence for amplification of the chicken NDRG4 gene by comparative genomics. We compared the corresponding human mRNA sequence with the predicted coding sequence of the chicken NDRG4 gene, and found that the assembled contig shared a high percentage of similarity with that of the human gene. PCR of samples from the ChickRH6 revealed the locations of the NDRG4 gene to be linked to the marker PARD6G (5 cR away) with a LOD score of 20.46. Our present results support the idea that the RH technique is indeed a powerful tool for mapping genes due to its rapidity, precision, convenience and reproducibility.

Keywords    chicken; genetic marker; mapping; NDRG4 gene; RH panel

A07089; Online publication date 25 January 2008; Received and accepted 10 August 2007

New Zealand Journal of Agricultural Research, 2007, Vol. 50: 767–770
0028–8233/07/5005–0767 © The Royal Society of New Zealand 2007

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