New Zealand Journal of Crop and Horticultural Science abstracts

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Factors affecting in vitro propagation of a Chinese wild grape (Vitis piasezkii var.pagnucii): shoot production and rhizogenesis

Jin Lin Zhang¹
Rui Xu²
Zi Yi Cao³
Suo Min Wang¹
Ji Zhou Ren¹

¹ Key Laboratory of Grassland Agro-ecosystems
 Ministry of Agriculture
 College of Pastoral Agricultural Science and Technology
 Lanzhou University
 P.O. Box 61, Lanzhou 730020
 People’s Republic of China
 email: smwang@lzu.edu.cn
² Gansu College of Traditional Chinese Medicine
 Lanzhou 730000
 People’s Republic of China
³ College of Agronomy
 Gansu Agricultural University
 Lanzhou 730070
 People’s Republic of China

Abstract Vitis piasezkii var. pagnucii is a Chinese wild grape species with high stress resistance, which could be used as a rootstock for wine grapes and table grapes. The present study was the first attempt for its in vitro micropropagation. Research was carried out regarding factors affecting shoot production and root initiation responses. 1⁄2 Murashige & Skoog (MS) medium supplemented with 0.2 mg/litre indoleacetic acid (IAA) resulted in the highest shoot production rate, earliest shoot development, most leaves, and least abnormal and etiolated leaves and shoots in the initial culture. 1⁄2 MS was the most suitable medium for rooting, shoot proliferation, and plantlet production of V. piasezkii var. pagnucii and Gansu (GS) medium was the second most suitable one. The highest rooting rate of shoots was obtained in 1⁄2 MS supplemented with 0.2 mg/litre IAA, 0.5 mg/litre indole-3-butyric acid (IBA) or 0.5 mg/litre a-naphthaleneacetic acid (NAA). Rooting rate, root numbers per shoot, and root length decreased when the three plant growth regulator (PGR) concentrations were higher or lower than those above. Pretreatments of dipping the bottom ends of microcuttings from shoots for 5 min in 100 mg/litre IBA and shading media enhanced rooting. Rooting and plantlet production rate and proliferation of shoots cultured under 2000 lux white light were higher than those under red light, and increased significantly with subsequent subculture.

Keywords Vitis piasezkii var. pagnucii; media; PGRs; in vitro micropropagation; rhizogenesis; shoot proliferation

New Zealand Journal of Crop and Horticultural Science, 2006, Vol. 34: 217–223
0014–0671/06/3403–0217      © The Royal Society of New Zealand 2006
H05095; Online publication date 6 July 2006. Received 17 August 2005; accepted 21 April 2006

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