New Zealand Journal of Crop and Horticultural Science abstracts

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Effect of desiccation, cold storage, and gibberellic acid on germination of somatic embryos in walnut (Juglans regia)

Bekir San

Department of Horticulture
Faculty of Agriculture
Suleyman Demirel University
Isparta, Turkey

Hatice Dumanoglu*

Department of Horticulture
Faculty of Agriculture
Ankara University
06110 Ankara, Turkey
email: dmanoglu@agri.ankara.edu.tr

Abstract The effect of desiccation, cold storage, gibberellic acid (GA₃ ), and various combinations of desiccation with GA₃ and cold storage treatments on root formation and germination of walnut (Juglans regia) somatic embryos originating from immature cotyledons of open-pollinated and non-pollinated seeds was investigated. Desiccation was effective on germination and cold storage (4 weeks at 4°C) or GA₃ (2.9, 8.6, 14.3, 20.0, and 25.7 µm) treatment together with desiccation improved the germination rate of walnut somatic embryos. The highest percentage of germination (69.1%) was determined with desiccated embryos originating from open-pollinated seeds of ‘Bilecik’ genotype on ‘Driver’ and ‘Kuniyuki’ walnut (DKW) basal medium containing 8.6 µm GA₃. The germination levels of embryos originating from both open-pollinated and non-pollinated seeds were found to be similar.

Keywords Juglans regia; root formation; plant regeneration; somatic embryogenesis

New Zealand Journal of Crop and Horticultural Science, 2007, Vol. 35: 73–78
0014–0671/07/3501–0073 © The Royal Society of New Zealand 2007
*Author for correspondence.

H06008; Online publication date 20 March 2007. Received 30 January 2006; accepted 28 August 2006

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