New Zealand Journal of Crop and Horticultural Science abstracts

Actinidia deliciosa leaf stomatal characteristics in relation to benzyladenine incubation periods in micropropagated explants

P. Moncaleán

Neiker-Tecnalia
Departamento de Producción y Protección Vegetal
Centro de Arkaute
Apartado 46
01080 Vitoria-Gasteiz, Spain
email: pmoncalean@neiker.net

B. Fernández
A. Rodríguez

Area Fisiología Vegetal
Instituto Universitario de Biotecnología
Departamento Biología de Organismos y Sistemas
Facultad de Biología
C/- Catedrático Rodrigo Uría
33071 Oviedo, Spain

Abstract Size, density, and stomata opening value in leaves of Actinidia deliciosa shoots cultured in three benzyladenine (BA) concentrations (4.4, 44, and 440 µM) during different incubation periods at the end of the multiplication, rooting, and acclimatisation phases were measured. A follow-up of the morphology of both adaxial and abaxial surfaces of the leaves was also performed. The presence or absence of 4.4 µm BA in the culture medium did not affect stomatal size during the multiplication phase, though the increase of the BA incubation period from 30 min to 4 h did induce an increment in the stomata transversal axis size. The application of BA (4.4 µM), regardless of the incubation period, increased stomatal density at the end of the multiplication phase. When the concentration of BA was increased in the culture medium during the multiplication phase, a decrease in the stomatal opening took place at the end of the acclimatisation phase.

Keywords Actinidia deliciosa; benzyladenine; micropropagation; leaf stomatal characteristics

New Zealand Journal of Crop and Horticultural Science, 2007, Vol. 35: 159–169
0014–0671/07/3501–0159      © The Royal Society of New Zealand 2007
H06060; Online publication date 12 April 2007. Received 26 June 2006; accepted 27 February 2007

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