New Zealand Journal of Agricultural Research abstracts
Occurrence, morphological characteristics and ribotyping of New Zealand
isolates of Duddingtonia flagrans, a candidate for biocontrol of animal
parasitic nematodes
R. A. Skipp
L. Y. Chen
AgResearch
Grasslands Research Centre
Private Bag 11 008
Palmerston North, New Zealand
G. W. Yeates
Landcare Research
Private Bag 11052
Palmerston North, New Zealand
T. R. Glare
AgResearch
P.O. Box 60
Lincoln New Zealand
Abstract The nematophagous fungus Duddingtonia flagrans
has shown potential for biological control of nematode parasites of livestock
in several countries. A survey for the presence of D. flagrans in
New Zealand was undertaken in 1997-98 on 24 farm, research, and domestic
properties in the southern North Island. The nematophagous fungi found on
isolation plates included species of Arthrobotrys, Dactylellina,Nematoctonus,
Harposporium, and Duddingtonia. Isolates that produced the three-dimensional
nets and abundant chlamydospores, characteristic of D. flagrans, were
detected in 6 of 205 samples examined. The fungus was obtained from a variety
of substrates such as cattle dung, horse dung, seepage from a silage pit,
and decomposing lawn clippings. Morphological characteristics, and the sequences
of ITS/5.8 region of ribosomal DNA, were very similar to those published
for D. flagrans. The fungus was re-isolated from the faeces of lambs
16-40 h after they had been dosed orally with chlamydospores, indicating
survival of gut passage.
Keywords gastro-intestinal nematodes; sheep; parasites;
nematophagous fungi; Duddingtonia flagrans; biological control
A01033 Received 18 October 2001; accepted 17 April 2002; published 30
September 2002
New Zealand Journal of Agricultural Research, 2002, Vol. 45: 187-196
0028-8233/02/4503-0187 $7.00/0 © The Royal Society of New Zealand
2002
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