New Zealand Journal of Agricultural Research abstracts
Developing tetraploid perennial ryegrass (Lolium perenne L.) populations
Ramakrishnan M. Nair
Agriculture Victoria Hamilton
Pastoral & Veterinary Institute
Private Bag 105, Hamilton
Victoria 3300, Australia
and
Cooperative Research Centre for Molecular Plant Breeding
Present address: South Australian Research & Development Institute
(SARDI), GPO Box 397, Adelaide, South Australia 5001.
Abstract In a breeding programme aimed at developing
tetraploids from Australian adapted germplasm, a root-tip squash technique
was initially used for ploidy determination. The highest recovery of tetraploids
(39.7%) was obtained by treatment of 1-week-old seedlings with 0.1% colchicine
concentration. Significant cultivar x colchicine concentration interaction
was observed in seedling survival, highlighting the difference in response
of the cultivars to colchicine treatment. Subsequently, flow cytometric estimation
of the nuclear DNA content in young leaf tissues was used to screen the C1
and C2 generations. The speed and the efficiency of this method
make it possible to examine a large number of plants. In the C1
population the percentage of tetraploids, diploids, and aneuploids was 25,
72.7, and 2.3% respectively, while in the C2 generation the percentage
of tetraploids, diploids, and aneuploids was 43, 2, and 55% respectively.
Keywords Lolium perenne; colchicine; tetraploid;
root-tip squash technique; flow cytometric technique
A03023;
Received 15 April 2003; accepted 18 August 2003; online publication date
26 February 2004
New Zealand Journal of Agricultural Research, 2004, Vol. 47: 45-49
0028-8233/04/4701-0045 © The Royal Society of New Zealand 2004
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