New Zealand Journal of Agricultural Research abstracts

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Somatic embryogenesis, callus production, and plantlet growth in sainfoin (Onobrychis viciifolia Scop.)

Nafiz Çelİktaş¹
Ersin Can¹
Ruştu Hatipoğlu²
Suleyman Avci¹

¹Department of Field Crops
Faculty of Agriculture
Mustafa Kemal University
31034 Antakya Hatay, Turkey
nafizcel@mku.edu.tr

²Department of Field Crops
Faculty of Agriculture
University of Çukurova
01330 Adana, Turkey

Abstract Plant parts (internode segments, young leaves, apical-axillary meristems, petioles and immature inflorescences) isolated from the fresh, full-leaved and juicy sainfoin (Onobrychis viciifolia Scop.) were used as explants in this study. The explants were cultured on B5 medium with 5.4 µM or 10.7 µM NAA combined with 0, 0.9, 1.4, or 2.3 µM of kinetin and 0, 0.9, 1.3, or 2.2 µM of BAP to determine the effects of explant types and medium compositions on the callus induction and plant regeneration. Somatic embryogenesis and organogenesis, as well as direct shoot regeneration from the explants, were observed depending on the explant types and medium composition. The effect of the phytohormone type and concentration on the callus induction rate was under explant dependency. The highest frequency of shoot regeneration was achieved from apical-axillary meristems on B5 medium with 10.7 µM NAA and 2.3 µM kinetin.

Keywords  sainfoin; phytohormones; explant; somatic embryogenesis

New Zealand Journal of Agricultural Research, 2006, Vol. 49: 383–388
0028–8233/06/4904–0383  © The Royal Society of New Zealand 2006

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