New Zealand Journal of Agricultural Research abstracts
Application of a biosensor for super-sensitive detector of
clenbuterol
Chen Cunshe
School of Chemical and Environmental Engineering
Beijing Technology and Business University
Beijing 100037, China
chencs@th.btbu.edu.cn
Li Xiaojuan
Information Engineering College
Capital Normal University
Beijing 100037, China
Abstract We explored the potential of the motor protein FoF1-ATPase
as biosensors of harmful residues in food. A fluorescence probe F1300
labelled in inner chromatophores was used as a pH indicator for
detecting proton flux driven by ATP synthesis in FoF1-ATPase.
Furthermore, the F1β subunit of FoF1-ATPase
was attached by a system of anti-β antibody-biotin-avidin-biotin-second
antibody (specific for clenbuterol) as a biosensor to detect
clenbuterol residues. The capturing mechanism was based on the
antibody-antigen reaction, while the detecting mechanism depended on a
fluorescence change responding to pH changes during the rotary
catalytic ATP synthesis process. The results showed that the activity
of FoF1-ATPase was affected with different loads
and the new biosensor may prove to be a useful nano-device for
super-sensitive (10–12 g/litre clenbuterol) detection.
Keywords biosensor; FoF1-ATPase;
molecular motors
A07079; Online publication date 21 December 2007; Received
and accepted 10 August 2007
New Zealand Journal of Agricultural Research, 2007, Vol. 50: 689–695
0028–8233/07/5005–0689 © The Royal Society of New Zealand 2007
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