New Zealand Journal of Agricultural Research abstracts
Application of comparative genomics in chicken gene mapping
Yong Tian1,2
Lizhi Lu2,*
Yan Fu1
Deqian Wang2
Guoqin Li2
Aiping Yuan2
Qingyan Yuan2
Jin Zhao3
1Laboratory of Animal Genetics, College of Animal
Sciences
Zhejiang University
268 Kaixuan Road
Hangzhou 310029, PR China
2Institute of Animal Husbandry and Veterinary Science,
Zhejiang Academy of Agricultural Sciences
198 Shiqiao Road
Hangzhou 310021, PR China
3College of Life Sciences, China Jiliang University
258 Xueyuan Road
Hangzhou 310018, PR China
*Author for correspondence: lulizhibox@163.com
Abstract The NDRG4 gene is a
member of the N-myc
downregulated gene family which belongs to the alpha/beta hydrolase
superfamily. The protein encoded by this gene is a cytoplasmic protein
that may be involved in the regulation of mitogenic signalling in
vascular smooth muscle cells. To map this gene in the chicken
chromosome, a 6000 rads chicken-hamster radiation hybrid panel
(ChickRH6) was used. Primers were designed according to the published
human sequence for amplification of the chicken NDRG4 gene by
comparative genomics. We compared the corresponding human mRNA sequence
with the predicted coding sequence of the chicken NDRG4 gene,
and found that the assembled contig shared a high percentage of
similarity with that of the human gene. PCR of samples from the
ChickRH6 revealed the locations of the NDRG4 gene to be linked
to the marker PARD6G (5 cR away) with a LOD score of 20.46. Our
present results support the idea that the RH technique is indeed a
powerful tool for mapping genes due to its rapidity, precision,
convenience and reproducibility.
Keywords chicken; genetic marker; mapping;
NDRG4 gene;
RH panel
A07089; Online publication date 25 January 2008; Received and
accepted 10 August 2007
New Zealand Journal of Agricultural Research, 2007, Vol. 50:
767–770
0028–8233/07/5005–0767 © The Royal Society of New Zealand 2007
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