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New Zealand Journal of Botany abstractsVesicular-arbuscular mycorrhizas in white clover: a scanning electron microscope and X-ray microanalytical studyGraham D. WalkerPhysics and Engineering Laboratory, DSIR, Private Bag, Lower Hutt, New Zealand
Conway Ll. PowellRuakura Agricultural Research Centre, Ministry of Agriculture and Fisheries, Private Bag, Hamilton, New ZealandAbstract Seedlings of white clover {Trifolium repens L.) were inoculated with several strains of mycorrhizal fungi and were cultured for sufficient time to allow the fungal endophytes to develop and mature. The roots were then subjected to a simple freeze-fracture, freeze-dry procedure in preparation for scanning electron microscopy and X-ray microanalysis. The morphology of the endophytes was observed and qualitative X-ray data were obtained indicating an accumulation of elements within mature arbuscles. Evidence of endophyte breakdown, accompanied by a reduction of the expected phosphorus concentrations, was also obtained and its implications are discussed.
Received 1 May 1978
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