New Zealand Journal of Botany abstract
Cytokinins and the regulation of plant form in three species of
Sophora
FIONA E. CARSWELL
JAMIE S. DAY*
KEVIN S. GOULD
Plant Science Group
School of Biological Sciences
The University of Auckland
Private Bag 92019
Auckland, New Zealand
*Present address: Department of Biochemistry, University of Otago, P.O. Box 56,
Dunedin, New Zealand.
PAULA E. JAMESON
Department of Botany
University of Otago
P.O. Box 56
Dunedin, New Zealand
Author for correspondence.
Present address: Department of Plant Biology and Biotechnology, Massey
University, Private Bag 11222, Palmerston North, New Zealand.
Abstract Possible relationships between cytokinins and
juvenile form of Sophora species were investigated. Exogenous
applications of 6-benzylaminopurine (6-BAP) to seedling S. microphylla
resulted in a more divaricating appearance. Treated plants had more outgrown
branches, wider branch angles, and narrower node angles than control plants.
6-BAP at 50 umol/l concentration was more effective in promoting this
change than at a concentration of 150 umol/l. The form of S.
prostrata was not altered by exogenous application of cytokinin. Endogenous
levels of storage and active cytokinins were assayed in pot-grown material,
using high performance liquid chromatography and radioimmunoassay. Compounds
with retention times and antibody specificity typical of known cytokinins in
other species, were detected in S. microphylla, S. prostrata, and
S. tetraptera. The recovery rates were low (1.5-18%) and results are
difficult to interpret because of large amounts of an unknown cytokinin.
However, it appears that the ratio of active to storage cytokinin is greater in
the divaricating than non-divaricating forms.
Keywords Sophora microphylla; Sophora
prostrata; Sophora tetraptera; cytokinin; divaricating habit
Abbreviations 6-BAP = 6-benzylaminopurine, cZ =
cis-zeatin, cZR = cis-9-ß-D-ribofuranosylzeatin, dZ =
dihydrozeatin, dZ9G = 9-ß-D-glucopyranosyl-(dihydro) zeatin, dZR =
9-ß-D-ribofuranosyl-(dihydro) zeatin, HPLC = high performance liquid
chromatography, iP = N6-([[Delta]]2-isopentenyl) adenine, iPA =
9-ß-D-ribofuranosyl-N6-([[Delta]]2-isopentenyl) adenine, iP9G
= 9-glucopyranosyl-N6-([[Delta]]2-isopentenyl) adenine, ms-iP =
2-methylthio-N6-([[Delta]]2-isopentenyl) adenine, RIA =
radioimmunoassay, Z = zeatin, Z9G = 9-ß-D-glucopyranosylzeatin, ZR =
9-ß-D-ribofuranosylzeatin
B94040
Received 12 September 1994; accepted 20 October 1995
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