New Zealand Journal of Botany abstract
B96058
Received 9 September 1996; accepted 18 April 1997
Antifungal proteins from seeds of Australian native plants and isolation of an
antifungal peptide from Atriplex nummularia
D. I. LAST
D. J. LLEWELLYN
CSIRO Division of Plant Industry
G. P. O. Box 1600
Canberra City
A.C.T. 2601, Australia
Abstract Extracts from seeds of 69 different species of
plants mostly originating from Australia were screened for antifungal agents
with activity against the plant pathogens, Fusarium oxysporum fsp.
vasinfectum, Rhizoctonia solani, Thielaviopsis
basicola, and Verticillium dahliae. Many seed extracts showed
inhibitory activity against one or more of the fungi tested. High antifungal
activities were detected in the genera Archontophoenix,
Atriplex, Alphitonia, Callistemon, and Dodonaea. Only
the Atriplex seed extract was shown to contain a protease-sensitive
antifungal activity. A 30 amino acid residue peptide (An1) was purified to
homogeneity from the seeds of Atriplex nummularia and its in
vitro activity against several fungal pathogens of plants was
characterised. Amino acid sequence analysis revealed An1 to be homologous to
the chitin-binding antimicrobial peptides of Amaranthus caudatus.
Keywords Australian flora; antifungal; seed protein;
Atriplex; antimicrobial peptide
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