New Zealand Journal of Botany abstract
Lipoxygenase activity in seeds from New Zealand native plants
CATHERINE L. ALLEN
JANE E. LANCASTER*
New Zealand Institute for Crop & Food Research Ltd
Private Bag 4704
Christchurch, New Zealand
*Present address: AgriFood Solutions Ltd, Voss Rd, RD4,
Christchurch, New Zealand.
DAVID S. ROBINSON
Procter Department of Food Science
University of Leeds
Leeds LS2 9JT, UK
Abstract Seeds from 48 New Zealand native plants, were tested
for lipoxygenase enzymes and subsequent formation of products from linoleic and
[[alpha]]-linolenic acid. Lipoxygenases are of interest to the food and
biotechnology industries as a means of producing natural flavour substances.
Extracts of
Gunnera hamiltonii possessed the highest lipoxygenase
activity of 5.6 umol min
-1 g
-1. Analysis of the 13-
and 9-positional isomers of the oxidation products of linoleic acid showed that
Melicystus lanceolatus x
M. flexuosa,
Pseudopanax
arboreus,
Carmichaelia kirkii,
Dianella nigra, and
Olearia
paniculata produced higher proportions of the 13-derivatives.
Hoheria
populnea,
Solanum laciniatum, and
Urtica australis produced
predominately the 9-isomers. Analysis of the 13- and 9-positional isomers of
the oxidation products of [[alpha]]-linolenic acid showed that all species
except
Chionochloa flavescens were able to oxidise the trienoic acid to
form 13-positional isomers.
Olearia ilicifolia produced only
13-positional isomers. Only 11 of the species produced substantial amounts of
the 9-derivatives from [[alpha]]-linolenic acid.
Chionochloa flavescens
showed only the activity of a 9-lipoxygenase.
Keywords lipoxygenases; polyunsaturated fatty acids;
hydroperoxide lyases; 9- and 13-positional isomers of
hydroperoxy-octadecadienoic acid; hydroxy-octadecadienoic acid;
hydroperoxy-octadecatrienoic acid; hydroxy-octadecatrienoic acid
B99013
Received 10 March 1999; accepted 21 June 1999
PDF file of entire paper: medium quality (660K); (scanned from paper original: notes about this process)
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