New Zealand Journal of Botany abstracts
The use of molecular techniques to resolve relationships among traditional
weaving cultivars of Phormium
Kim McBreen*
Peter J. Lockhart
Patricia A. McLenachan
Institute of Molecular BioSciences
Massey University
Private Bag 11-222
Palmerston North, New Zealand
*Present address: Biological Sciences, University of Missouri, 226 Tucker
Hall, Columbia, Missouri 65211, USA.
Email: kmcbreen@yahoo.com
Sue Scheele
Landcare Research
P.O. Box 69
Lincoln 8152, New Zealand
Alastair W. Robertson
Institute of Natural Resources
Massey University
Private Bag 11-222
Palmerston North, New Zealand
Abstract Molecular techniques - sequencing of the nuclear
internal transcribed spacer region (ITS), and fingerprinting of amplified
fragment length polymorphisms (AFLP) and intersimple sequence repeats (ISSR)
- were investigated for their potential to characterise genetic variation
in harakeke (Phormium spp., family Hemerocallidaceae) in New Zealand.
ITS showed no variation between species of Phormium. However, both
AFLP and ISSR identified genome regions that were polymorphic among intraspecific
accessions of Phormium obtained from the National New Zealand Flax Collection.
When used as a direct sequencing marker, one AFLP fragment distinguished
two major groups of nuclear haplotypes. The partitioning of taxa into these
groups was supported by phylogenetic analysis of ISSR fingerprinting profiles.
Split decomposition, a network-building technique, was shown to be useful
in representing the sorts of relationships present in this study of intraspecific
variation. Further sampling of natural populations is required to understand
the origins and present-day distributions of these accessions; however, the
present results show the usefulness of these methods for addressing such
ethnobotanic questions.
Keywords ethnobotany; ITS; ISSR; AFLP; marker systems;
phylogeny; SCAR markers; Phormium; harakeke; split decomposition
B02010 Received 11 February 2002; accepted 20 December 2002; published
11 June 2003
New Zealand Journal of Botany, 2003, Vol. 41: 301-310
0028-825X/03/4102-0301 $7.00 © The Royal Society of New Zealand
2003
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