New Zealand Journal of Botany abstracts
Peroxidase and polyamine activity variation during the in vitro
rooting of Berberis buxifolia
Miriam Elisabet Arena
Guillermo Martínez Pastur
Laboratorio de Propagación y Producción Vegetal
Centro Austral de Investigaciones Científicas (CADIC-CONICET)
Cc 92 (9410) Ushuaia
Tierra del Fuego, Argentina
Email: arena@satlink.com
María Patricia Benavides
Facultad de Farmacia y Bioquímica
Universidad de Buenos Aires (UBA)
Junín 956 (1113)
Buenos Aires, Argentina
Diego Zappacosta
Departamento de Agronomía
Universidad Nacional del Sur (UNSur)
San Andrés 800 - Altos del Palihue (8000)
Bahía Blanca, Argentina
Eleonora Eliasco
Laboratorio de Propagación y Producción Vegetal
Centro Austral de Investigaciones Científicas (CADIC-CONICET)
Cc 92 (9410) Ushuaia
Tierra del Fuego, Argentina
Néstor Curvetto
Departamento de Agronomía
Universidad Nacional del Sur (UNSur)
San Andrés 800 - Altos del Palihue (8000)
Bahía Blanca, Argentina
Abstract Berberis buxifolia is a Patagonian
shrub with great economic potential for tinctorial, pharmacological, and
food industries. Clonal propagation is possible through in vitro culture
and is also useful for metabolite production. However, this species is difficult
to root, and to improve this, more knowledge of rhizogenesis processes is
needed. Polyamines and peroxidases are useful biochemical markers during
analysis of rooting phases for correlation with tissue morphological changes.
Therefore, endogenous polyamine (putrescine, spermidine, spermine) changes,
peroxidase activity evolution, and morphological development were studied
to characterise the in vitro rhizogenesis of microshoots of B.
buxifolia and, thus, to define the rooting phases. Polyamine and peroxidase
changed significantly during the rooting period, and had opposite behaviours
which were directly related to the IBA media content. The lower polyamine
concentration and the higher peroxidase activity were found in a treatment
with a dark period during the first four days and with IBA in the culture
medium. Putrescine was the most abundant polyamine found in B. buxifolia
tissues, 14- to 18-fold more than spermidine and spermine, respectively.
Therefore, these compounds were used to define the rooting phases: an induction
phase (0 to 4-7 days) followed by an expression phase (4-7 to 28 days). The
observed changes in the biochemical markers could be correlated with microscopic
and macroscopic tissue observations in the microshoots, and the time course
of rooting percentage. Successive culture media can be developed including
polyamines, or other compounds and environmental conditions, which positively
modify the studied biochemical markers behaviour.
Keywords rooting markers; rhizogenesis; histology;
micropropagation; Patagonia; Berberis buxifolia
B02077 Received 6 December 2002; accepted 15 May 2003; online publication
date 11 September 2003
New Zealand Journal of Botany, 2003, Vol. 41: 475-485
0028-825X/03/4103-0475 $7.00 © The Royal Society of New Zealand
2003
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