New Zealand Journal of Botany
abstracts
Histological events during in
vitro rooting of Nothofagus
nervosa (Fagaceae)
Guillermo
Martínez
Pastur
Miriam Arena
Centro Austral de
Investigaciones Científicas (CONICET)
cc 92 (9410)
Ushuaia, Tierra del Fuego, Argentina
Luis Hernandez
Departamento de
Agronomía
Universidad Nacional del Sur
Altos del Palihue (8000)
Bahía Blanca, Buenos Aires, Argentina
and
Comisión
de Investigaciones
Científicas (CIC)
Calle 526 e/ 10 y 11
(1900) La Plata, Buenos Aires, Argentina
Néstor
Curvetto
Departamento de
Agronomía
Universidad Nacional del Sur
Altos del Palihue
(8000) Bahía Blanca, Buenos Aires, Argentina
and
Centro de Recursos Naturales
Renovables de la Zona
Semiárida (CERZOS
– CONICET)
Km 7, Camino a la Carrindanga
(8000) Bahía Blanca, Buenos Aires, Argentina
Eleonora Eliasco
Centro Austral de
Investigaciones Científicas (CONICET)
cc 92 (9410)
Ushuaia, Tierra del Fuego, Argentina
Abstract In
vitro rooting protocols were
previously performed on Nothofagus
nervosa to optimise the culture
medium and environmental conditions. A qualitative and quantitative
tissue differentiation study during adventitious rooting in N.
nervosa under different
light/dark conditions was carried out to increase knowledge of the
rhizogenesis process in shoots. The histological events through the
rooting period were consistent with the proposed biochemical markers
(peroxidases, tissue nutrient contents, polyamines, flavonoids).
Development of parenchymatic tissues, shown by the staining pattern of
the cell contents, and cambium activity were the best tissues to define
the rooting phases. Knowledge of the histological changes could be used
to optimise a protocol for micro-propagation improving timing,
quantity, and quality of roots, which would positively affect the
survival rate during hardening.
Keywords rooting
phases; cambium activity; root anatomy; histo-chemistry; peroxidase
activity
B04020; Received 8 June 2004;
accepted 31 August 2004; Online publication date 17 March 2005
New Zealand Journal of Botany,
2005, Vol. 43: 61–70
0028–825X/05/4301–0061 © The Royal Society of New Zealand 2005
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