New Zealand Journal of Botany
abstracts
Polyamines and inhibitors used
in successive culture media for in
vitro rooting in Berberis
buxifolia
Miriam Elisabet Arena
Guillermo Martínez Pastur
Laboratorio de
Propagación y Producción
Vegetal
Centro Austral de Investigaciones Científicas (CADIC-CONICET)
Cc 92 (9410) Ushuaia
Tierra del Fuego, Argentina
arena1@infovia.com.ar
María
Patricia Benavides
Facultad de Farmacia y
Bioquímica
Universidad de Buenos Aires (UBA)
Junín 956 (1113)
Buenos Aires, Argentina
Néstor
Curvetto
Departamento de
Agronomía
Universidad Nacional del Sur (UNSur)
San Andrés 800 – Altos del Palihue (8000)
Bahía Blanca, Argentina
Abstract Although
Berberis buxifolia
has been reported to propagate in
vitro, rooting is difficult to
achieve during subcultures, with reduced quantity and quality of roots,
as is the case with many other woody species. Several cofactors of
rhizogenesis have been proposed, and the role of polyamines in rooting
has recently acquired relevance. Polyamines can stimulate or inhibit
microshoot rooting depending on the type and concentration of polyamine
and rooting phase, while polyamine inhibitors can improve rooting in
some cases. A study of the in
vitro rooting of B.
buxifolia using polyamines and a
mix of polyamine inhibitors in a two-step culture medium is described
here, in which a new successive rooting medium was successfully
implemented. While polyamine inhibitors sometimes improved rooting,
nutrient medium containing a low polyamine concentration (i.e., 1
µM)
enhanced rooting compared with the control medium. The best microshoot
rooting response resulted from the addition of 1 µM
spermidine during the expression phase in the absence of inhibitors in
both rooting phases. Overall, incorporating polyamine in the successive
media enhances the quality and quantity of roots in B.
buxifolia, thus confirming their
role during the induction and expression of the radicle primordia
differentiation.
Keywords calafate;
micropropagation; Patagonia; rhizogenesis; polyamine inhibitors; root
system quality; Berberis buxifolia
B04035; Received 3 September
2004; accepted 12 January 2005; Online publication date 26 April 2005
New Zealand Journal of Botany,
2005, Vol. 43: 373–380
0028–825X/05/4302–0373 © The Royal Society of New Zealand 2005
PDF file of entire paper: Print-quality
(275K) | screen-quality
(347K)
This year's abstracts
|
Journal
home page |
All abstracts
|
Publishing
home page
