New Zealand Journal of Botany abstracts
Species relationships and genetic variation in the New Zealand
endemic Leucogenes (Asteraceae: Gnaphalieae)
R. D. Smissen
I. Breitwieser
Allan Herbarium
Landcare Research
PO Box 40
Lincoln 7640, New Zealand
Abstract AFLP profiles, nuclear ITS sequences, and
chloroplast psbA-trnH intergenic spacer sequences for
representative samples of the New Zealand edelweiss species Leucogenes
grandiceps, L. leontopodium, L. neglecta, and
L. tarahaoa were examined. Analysis of AFLP profiles from a small
number of populations strongly groups samples of each species, and in
turn samples of diploid (2n = 2x = 28) L.
leontopodium and tetraploid (2n = 4x = 56) L.
neglecta cluster together, consistent with a derivation of the
latter from the former by autopolyploidy. However, octoploid (2n =
8x = 112) L. tarahaoa does not cluster closely with
either L. neglecta or L. leontopodium, indicating
this species has an independent origin. Analyses of chloroplast DNA
sequences show that although the majority of Leucogenes samples
share similar chloroplast sequences not sampled from other species of
New Zealand Gnaphalieae, a single accession of L. grandiceps
and North Island populations of L. leontopodium share
chloroplast sequences more similar to other species of New Zealand
Gnaphalieae. ITS sequence variation is more complex, with sequences
sampled from Leucogenes not constituting a monophyletic group,
and striking intraspecific variation detected within L. grandiceps.
Keywords Leucogenes; Raoulia; Gnaphalieae;
Asteraceae; ITS; psbA-trnH; intraspecific polymorphism;
trans-specific polymorphism; AFLP; phylogenetic incongruence; lineage
sorting; introgression; polyploidy; DNA bar-coding; New Zealand flora
B07021; Online publication date 25 February 2008; Received 2 July
2007; accepted 21 December 2007
New Zealand Journal of Botany, 2008, Vol. 46: 65–76
0028–825X/07/4601–0065 © The Royal Society of New Zealand 2008
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