New Zealand Journal of Crop and Horticultural Science abstracts
Transgenic resistance genes from nepoviruses: efficacy and other properties
J. I. COOPER
School of Biological and Environmental
Sciences
Murdoch University
Perth, Western Australia 6150
M. L. EDWARDS
O. ROSENWASSER
Natural Environment Research Council
Institute of Virology and Environmental
Microbiology
Mansfield Road
Oxford OX1 3SR, United Kingdom
N. W. SCOTT
Department of Applied Biology and
Biotechnology
De Montfort University
Leicester, United Kingdom
Abstract Evidence of RNA-RNA recombination or
transcapsidation was sought in transgenic tobacco (
Nicotiana tabacum L.
`Xanthi-nc') containing the 3'-non coding sequence (1.3 kb) from a birch
isolate of cherry leaf roll nepovirus (CLRV). Following mechanical inoculation
with a rhubarb isolate of the virus, no evidence of recombination was obtained
using polymerase chain reactions whether the transgenic gene produced
transcripts in the sense or the antisense orientation. Transgenic tobacco
containing the CLRV-derived sequence (in either orientation) did not lessen the
pathological effects of the rhubarb isolate of the virus although there was
amelioration of the disease caused by the birch isolate. When expressed as a
transgenic gene in tobacco, the capsid coding sequence of arabis mosaic
nepovirus (ArMV) lessened infectibility by nematodes carrying that virus. This
construct did not influence the pathogenicity of potato virus Yo (PVYo),
tobacco rattle tobravirus (PRN), alfalfa mosaic ilarvirus (AlMV), or CLRV, did
not alter the levels of their accumulation and did not render their virions
more or less prone to recognition by ArMV-specific antibodies. Uninoculated
tobacco expressing the ArMV capsid protein contained ArMV-like particles which
co-purified with RNA that hybridised with cDNA to tobacco mRNA and to DNA
complementary to the capsid coding sequence.
Keywords Nicotiana tabacum; arabis mosaic nepovirus,
cherry leaf roll nepovirus; recombination; transcapsidation; psuedovirions;
Agrobacterium tumefaciens
PDF file of entire paper: medium quality (826K); (scanned from paper original: notes about this process)
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