New Zealand Journal of Crop and Horticultural Science abstracts
Shoot regeneration from leaf discs of Limonium perigrinum using
thidiazuron
JOHN F. SEELYE
DEBRA J. MADDOCKS*
GARRY K. BURGE
ED R. MORGAN
New Zealand Institute for Crop & Food
Research Ltd
Levin Research Centre
Private Bag 4005
Levin, New Zealand
*Present address: Carter Holt Harvey Ltd, Forest Biotechnology Centre, P.O. Box
2463, Rotorua, New Zealand.
Abstract Shoots were regenerated from 12-mm diameter leaf discs
derived from greenhouse plants of Limonium perigrinum (Bergius), with
either zeatin or thidiazuron (TDZ), the latter producing five times the number
of shoots. After 8 weeks exposure to a modified Murashige and Skoog basal
medium (BM) supplemented with 3.0 mg/litre TDZ, an average of 13 shoots/disc
formed, 90% of which were <= 1 mm long. There was no significant difference
in the total number of shoots formed at 1.0-5.0 mg/litre TDZ, however at lower
rates (1.0-2.0 mg/litre) a greater proportion of longer shoots (> 1 mm)
developed. Shoots regenerated after 8 weeks from leaf discs exposed to BM with
TDZ for from 2 h to 6 weeks before being transferred to growth-regulator-free
medium. A mean of 4.8 shoots/disc formed from the shortest TDZ pulse period,
with the majority being longer than 1 mm. Serial sections of discs pulsed with
TDZ medium showed many suppressed shoot initials within the new tissue
originating from the cut edge of the discs.
Keywords Limonium; regeneration; thidiazuron;
transformation; tissue culture; pulsing
PDF file of entire paper: medium quality (1415K); (scanned from paper original: notes about this process)
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