New Zealand Journal of Crop and Horticultural Science abstracts
In vitro long-term storage of asparagus
P. J. FLETCHER
New Zealand Institute for Crop & Food
Research Limited
Private Bag 4704
Christchurch, New Zealand
Abstract To assist with commercial production of asparagus
(Asparagus officinalis L.) clones, a system for in vitro, long-term cold
storage of high health asparagus germplasm was developed. In vitro mini-crowns,
with and without storage roots, were stored on a medium consisting of Murashige
and Skoog salts with the addition of 3% sucrose, 4% sorbitol, 0.4 mg/litre
thiamine, 200 mg/litre glutamine, 100 mg/litre inositol, 1.0 mg/litre
ancymidol, and 0.8% agar. The cultures were incubated at 6deg.C with a
photoperiod of 16 h (light level of 70-90 umol/m2 per s). The role
of sorbitol was examined, as was the value of photoperiod. After 16 months of
storage, the viability and re-culturing ability of the in vitro cultures was
assessed. To date, the cultures have survived 2 years of storage with 100%
success in regrowth to their original growth form in culture and on transfer to
glasshouse conditions.
Keywords long-term cold storage; in vitro culture;
Asparagus officinalis L.; germplasm; mini-crown; sorbitol; osmotic
stress; viability
New Zealand Journal of Crop and Horticultural Science, 1994, Vol. 22:
351-359
0114-0671/94/2204-0351 $2.50/0 (c) The Royal Society of New Zealand
1994
PDF file of entire paper: medium quality (2951K); (scanned from paper original: notes about this process)
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