New Zealand Journal of Crop and Horticultural Science abstracts
Transformation studies of Actinidia chinensis Planch.
LENA G. FRASER
JACQUELINE KENT
CATHERINE F. HARVEY
The Horticulture and Food Research
Institute of New Zealand
Mt Albert Research Centre
Private Bag 92 169
Auckland, New Zealand
Abstract Transformed plants of four different genotypes of
Actinidia chinensis Planch. were recovered from experiments using the
Agrobacterium tumefaciens strains A281 and C58, both carrying the binary
vector pKIWI105.
A. chinensis was compared to a related species
A.
deliciosa, in its amenability to transformation and regeneration
procedures. During regeneration
A. chinensis shoot initiation was more
rapid and prolific than
A. deliciosa, although
A. chinensis was
more sensitive to small changes in growth regulator concentration. Comparisons
between the different genotypes of
A. chinensis showed only minor
differences in growth regulator preferences. The strains of
Agrobacterium
tumefaciens that were effective as gene transfer agents in
A. chinensis
were the same as those which transformed
A. deliciosa. Southern
analysis and Polymerase Chain Reaction amplification of the transferred genes
were used to confirm the transgenic nature of regenerated plantlets.
Keywords Actinidia chinensis; Actinidia
deliciosa; kiwifruit; regeneration; transformation
New Zealand Journal of Crop and Horticultural Science, 1995, Vol. 23:
407-413
0114-0671/95/2304-0407 $2.50/0 (c) The Royal Society of New Zealand
1995
PDF file of entire paper: medium quality (1380K); (scanned from paper original: notes about this process)
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