New Zealand Journal of Crop and Horticultural Science abstracts

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Towards the transformation of onions (Allium cepa)

C. C. EADY

New Zealand Institute for Crop & Food
Research Limited
Private Bag 4704
Christchurch, New Zealand

Abstract  This review outlines the potential for developing a reliable transformation system for onions (Allium cepa). A summary of the onion tissue culture literature is presented along with descriptions of the gene transfer systems available. The most suitable regulatory sequences and selective genes that could be used for onion transformation are described, together with the problems which may be encountered following the integration into the particularly large genome of onion. The review proposes likely routes by which transformation and regeneration may be achieved, and suggests possible characteristics of onions which may be altered using such a technique.

Keywords  Allium cepa; onion; Agrobacterium tumefaciens; particle gun; transformation; tissue culture; transgenic plants

New Zealand Journal of Crop and Horticultural Science, 1995, Vol. 23: 239-250

0114-0671/95/2303-0239 $2.50/0 (c) The Royal Society of New Zealand 1995

Review

PDF file of entire paper: medium quality (1066K); (scanned from paper original: notes about this process)

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