New Zealand Journal of Crop and Horticultural Science abstracts

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A stress-, pathogenesis-, and allergen-related cDNA in apple fruit
is also ripening-related

ROSS G. ATKINSON¹
JOHANNA PERRY¹
TOSHIYUKI MATSUI²
GAVIN S. ROSS¹
ELSPETH A. MACRAE¹

1Horticulture and Food Research
Institute of New Zealand
Mt Albert Research Centre
Private Bag 92 169
Auckland, New Zealand

2Department of Agroindustrial Science
Faculty of Agriculture, Kagawa University
Miki-cho, Kagawa 761-07
Japan

Abstract  The screening of a ripe apple (Malus domestica `Golden Delicious') cDNA expression library with polyclonal antibodies to spinach sucrose-phosphate synthase resulted in the isolation of a cDNA clone pAP15. This clone encodes a homologue of a class of stress and pathogenesis-related clones, and is also highly homologous to a class of allergenic proteins from apple without known biological function. The pAP15 cDNA insert is 817 base pairs in length and codes for a protein of 17.5 kD. Genomic Southern analysis suggests that the gene belongs to a family of at least 15 members. Northern analysis indicates that pAP15 is ripening-related, with an accumulation of homologous mRNA coincident with ethylene production during apple fruit ripening.

Keywords  allergen; apple; pathogenesis-related; ripening; stress

New Zealand Journal of Crop and Horticultural Science, 1996, Vol. 24: 103-107

0114-0671/96/2401-0103 $2.50/0 (c) The Royal Society of New Zealand 1996

Short communication

PDF file of entire paper: medium quality (1862K); (scanned from paper original: notes about this process)

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