New Zealand Journal of Crop and Horticultural Science abstracts
Tissue culture of Ranunculus lyallii Hook. f.
R. A. BICKNELL
R. H. BRAUN
New Zealand Institute for Crop & Food
Research Limited
Private Bag 4704
Christchurch, New Zealand
A. C. EVANS
New Zealand Institute for Crop & Food
Research Limited
Private Bag 50 034
Mosgiel, New Zealand
E. R. MORGAN
New Zealand Institute for Crop & Food
Research Limited
Private Bag 4005
Levin, New Zealand
Abstract A micropropagation method is presented for
Ranunculus lyallii Hook. f. Seedling establishment in culture required
surface sterilisation of the achene, followed by dissection of the embryo from
the other tissues of the seed. This process was necessary both to avoid the
persistent presence of the fungus Botrytis cinerea and to overcome
physiological dormancy. Seedlings germinated and grew well on an
agar-solidified basal medium comprising half-strength Murashige and Skoog (MS)
salts (Murashige & Skoog 1962), MS organics, and 3% sucrose. Shoot
proliferation increased with increasing 6-benzylaminopurine (BAP) concentration
up to 0.6 mg/litre, whereas higher rates caused shoot distortion and inhibited
subsequent rooting of plantlets. Shoot proliferation was optimised on basal
medium supplemented with 0.2 mg/litre BAP. Root formation was completely
inhibited by BAP, even at 0.1 mg/litre, the lowest concentration tested. Adding
0.1-0.5 mg/litre indole-3-butyric acid (IBA) to the medium had no effect on
this response. As shoots readily formed roots on the basal medium without
additional growth regulators, this formulation was used for rooting in all
subsequent manipulations.
Keywords Ranunculus lyallii; micropropagation
New Zealand Journal of Crop and Horticultural Science, 1996, Vol. 24:
303-306
0114-0671/96/2404-0303 $2.50/0 (c) The Royal Society of New Zealand
1996
PDF file of entire paper: medium quality (271K); (scanned from paper original: notes about this process)
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