New Zealand Journal of Crop and Horticultural Science abstracts
In vitro propagation of Gentiana cerina and Gentiana
corymbifera
E. R. MORGAN
New Zealand Institute for Crop & Food
Research Limited
Levin Research Centre
Private Bag 4005
Levin, New Zealand
R. M. BUTLER
R. A. BICKNELL
New Zealand Institute for Crop & Food
Research Limited
P.O. Box 4704
Christchurch, New Zealand
Abstract The micropropagation of
Gentiana cerina and
G. corymbifera was investigated. Cultures were initiated from axillary
shoots or seed. Seeds of
G. corymbifera germinated on a Murashige &
Skoog (MS) medium containing 100 mg/litre gibberellic acid (GA3) with 54% of
the seed germinating within 70 days. In the absence of GA3 germination did not
exceed 5%. Both species proliferated shoots on MS medium supplemented with
6-benzylaminopurine (BAP). For
G. cerina there was no significant
difference in proliferation rates for BAP concentrations between 0.05 and 0.5
mg/litre. In contrast
G. corymbifera gave highest multiplication rates
on 0.2 mg/litre BAP. Addition of 1 mg/litre GA3 to the medium gave improved
proliferation compared to treatments in which GA3 was absent. The best
treatment for
G. cerina resulted in a shoot multiplication rate in
excess of 7-fold after 50 days whereas for
G. corymbifera this increase
was more than 3-fold. Root initiation occurred on MS medium supplemented with
indole-3-butyric acid (IBA). In both species the frequency of explants with
roots increased with increasing IBA concentration, but at IBA >= 1 mg/litre
and 3 mg/litre for
G. cerina and
G. corymbifera respectively a
high proportion of the plants developed a basal callus. Survival of the
G.
cerina plants during acclimatisation was related to IBA concentration but
survival was unrelated to IBA concentration for
G. corymbifera.
G.
cerina survival rates decreased from 80% with up to 0.3 mg/litre IBA to c.
5% at the highest rate of IBA. For
G. corymbifera average survival of
plants after acclimatisation was just over 20%. Thus root initiation with 0.3
mg/litre IBA can be recommended for both species.
Keywords Gentiana cerina; G. corymbifera;
micropropagation
New Zealand Journal of Crop and Horticultural Science, 1997, Vol. 25:
1-8
0114-0671/97/2501-0001 $2.50/0 (c) The Royal Society of New Zealand
1997
PDF file of entire paper: medium quality (645K); (scanned from paper original: notes about this process)
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