New Zealand Journal of Crop and Horticultural Science abstracts
Differentiation of Australasian potato cyst nematode (PCN) populations using
the polymerase chain reaction (PCR)
S. R. BULMAN
J. W. MARSHALL
New Zealand Institute for Crop & Food
Research Limited
Private Bag 4704
Christchurch, New Zealand
Abstract Molecular examination of the ribosomal internal
transcribed spacer (ITS) region in potato cyst nematodes (PCN) is described.
The ITS was amplified and sequenced from a number of PCN collections. A low
level of sequence variation was found between Globodera rostochiensis,
G. pallida, and a Peruvian PCN collection, but no variation within
Australasian collections of species was noted. Polymerase chain reaction (PCR)
primers based upon the G. rostochiensis-G. pallida sequence
differences were designed and successfully used to identify mixed PCN species
in a single PCR reaction.
Keywords PCN; Globodera rostochiensis; Globodera
pallida; ribosomal ITS; PCR; microheterogeneity
New Zealand Journal of Crop and Horticultural Science, 1997, Vol. 25:
123-129
0114-0671/97/2502-0123 $7.00/0 (c) The Royal Society of New Zealand
1997
PDF file of entire paper: medium quality (925K); (scanned from paper original: notes about this process)
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