New Zealand Journal of Crop and Horticultural Science abstracts
Comparison of RAPD and morpho-nut markers for revealing genetic
relationships between chestnut species (Castanea spp.) and New Zealand chestnut
selections
N. C . ORAGUZIE
Department of Plant & Microbial Sciences
University of Canterbury
Private Bag 4800
Christchurch, New Zealand
D. L. MCNEIL
Department of Plant Science
P. O. Box 84
Lincoln University
Christchurch, New Zealand
A. M. PATERSON
Department of Entomology
and Animal Ecology
P. O. Box 84
Lincoln University
Christchurch, New Zealand
H. CHAPMAN
Department of Plant & Microbial Sciences
University of Canterbury
Private Bag 4800
Christchurch, New Zealand
Abstract This study explored different character data sets
(random amplified polymorphic DNA (RAPD) and morpho-nut characters),
techniques, and systematic methodologies in an attempt to reconstruct the
phylogenetic relationships and origins of New Zealand chestnut (
Castanea
spp.) selections. The study was prompted by confusion regarding the
relationships of New Zealand chestnut selections and the introduced chestnut
species in New Zealand. RAPDs demonstrated that
C. dentata is not one of
the parents of the chestnut selections and revealed that `1002' and `1007'
could be a hybrid of
C. mollissima and
C. crenata species. A
comparison of the trees from RAPD and morpho-nut characters showed that the
trees were significantly congruent. The groupings of the chestnut species and
the New Zealand chestnut selections were based on their geographic locations.
The study also demonstrated that most of the New Zealand chestnut selections
were not hybrids of
C. sativa and
C. crenata but rather most of
the South Island selections were found to be
C. sativa-like whereas many
of the North Island selections were
C. crenata-like. It is suggested
that both RAPD and morpho-nut approaches be used in combination for the
determination of the phylogeny of New Zealand chestnuts.
Keywords Castanea species; New Zealand chestnut
selections; RAPD; morpho-nut; congruence; phylogeny
H97-43
Received 6 October 1997; accepted 15 March 1998
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