New Zealand Journal of Crop and Horticultural Science abstracts
Inheritance and expression of transgenes in kiwifruit
RAYMOND W. M. FUNG
BART-JAN JANSSEN
School of Biological Sciences
University of Auckland
Private Bag 92 019
Auckland, New Zealand
BRET A. MORRIS
The Horticulture and Food Research
Institute of New Zealand Ltd
Private Bag 92 169
Auckland, New Zealand
RICHARD C. GARDNER
School of Biological Sciences
University of Auckland
Private Bag 92 019
Auckland, New Zealand
Abstract The inheritance and expression of transgenes was
monitored in kiwifruit (
Actinidia deliciosa (A.Chev.) C.F. Liang et A.R.
Ferguson) established in a field trial. A gene encoding resistance to kanamycin
and a reporter gene expressing the [[beta]]-glucuronidase (GUS) enzyme were
both inherited and expressed in progeny of the kiwifruit plants. For two plants
examined in detail, molecular evidence suggested that there were multiple
copies of both the kanamycin resistance (
nptII)
gene and the
uidA gene. None of the actively expressing copies of
nptII or
uidA were linked. Expression of GUS was variable in some progeny plants,
consistent with the idea that gene silencing phenomena were operating.
Keywords Actinidia deliciosa; kanamycin resistance;
[[beta]]-glucuronidase; gene silencing; field trial
H97068
Received 23 December 1997; accepted 1 May 1998
PDF file of entire paper: medium quality (2661K); (scanned from paper original: notes about this process)
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