New Zealand Journal of Crop and Horticultural Science abstracts
Potato germplasm: in vitro storage and virus reduction
P. J. FLETCHER
J. D. FLETCHER
R. J. CROSS
New Zealand Institute for Crop & Food
Research Limited
Private Bag 4704
Christchurch, New Zealand
Abstract An efficient protocol for
in vitro long-term
storage of a potato (
Solanum tuberosum) germplasm collection has been
devised. Potato explants were prepared for tissue culture and then underwent
chemical (using the antiviral chemical, ribavirin) and heat therapy. It
combines previously reported protocols of
in vitro virus elimination,
micro tuber induction, and long-term storage and is especially suitable for
conserving a large collection. Five weeks later, the apical tips were
introduced to microtuber induction medium. After shoot and root development,
the tissue was ELISA tested for confirmation of significant virus reduction and
indexing, and then the plantlets were transferred to a cabinet set for
microtuber induction. The microtubers formed after 12-16 weeks and then the
pottles were transferred into long-term storage conditions. A minimum storage
time of 3 years has been achieved.
Keywords potato; germplasm; long-term cold storage; in
vitro culture; virus reduction; virus indexing; ribavirin
H97060
Received 3 December 1997; accepted 18 May 1998
Short communication
PDF file of entire paper: medium quality (803K); (scanned from paper original: notes about this process)
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