New Zealand Journal of Crop and Horticultural Science abstracts
Suppression of Botrytis cinerea sporulation on irradiated grape leaf
tissue by the antagonistic bacterium Serratia liquefaciens
S. A. WHITEMAN*
A. STEWART
Soil, Plant & Ecological Sciences Division
P. O. Box 84
Lincoln University
Canterbury, New Zealand
*Present address: The Horticulture and Food Research Institute of
New Zealand Ltd, Private Bag
92 169, Auckland, New Zealand. email:
swhiteman@hort.cri.nz
Abstract A range of bacteria were screened to identify
potential antagonists against Botrytis cinerea on grape tissue (Vitis
vinifera L.). From dual culture tests, 13 isolates were selected which
exhibited antagonism towards B. cinerea mycelial growth. Inhibition
zones ranged between 3 and 12 mm. These isolates were screened in a grape
leaf bioassay. Irradiated leaf discs were inoculated with B.
cinerea (1 x 104 spores/ml) then challenged with bacteria
applied at 1 x 108 colony forming units (cfu)/ml in tryptic soya
broth (TSB). Sporulation of B. cinerea was significantly
(P = 0.05) reduced on leaf discs treated with Serratia
liquefaciens isolates B47 and 171B by 62 and 57% respectively, 7 days after
application. In a second leaf bioassay, B47 applied in TSB significantly
(P = 0.05) reduced B. cinerea sporulation by 65% after 21
days compared to a 39% suppression achieved with isolate 171B (applied in TSB)
and the fungicide, iprodione. However, when applied in Ringers solution, both
isolates failed to reduce B. cinerea sporulation. This research has
identified two isolates of S. liquefaciens able to suppress sporulation
of B. cinerea on grape tissue and has shown that bacterial
application solution can strongly influence the degree of suppression
achieved.
Keywords bacteria; biological control; Botrytis
cinerea; dual plate assay; grape; iprodione; leaf disc assay; mycelial
growth; Serratia liquefaciens; sporulation
H98022
Received 14 May 1998; accepted 7 August 1998
Short communication
PDF file of entire paper: medium quality (549K); (scanned from paper original: notes about this process)
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