New Zealand Journal of Crop and Horticultural Science abstracts
Improving main agronomic characters of dihaploids with 2n gametes by means
of sexual hybridisation and anther culture of potato (Solanum
tuberosum)
D. WANG
Y. RAN*+
Agrobiotechnological Engineering Institute
Gansu Agricultural University
Lanzhou, Gansu 730070
People's Republic of China
email:wangdi@sun2.gsau.edu.cn
*Present address: School of Biology Science, The University of
Auckland, Private Bag 92 019, Auckland, New Zealand.
email:y.ran@auckland.ac.nz
Abstract Three hybrid populations were obtained from 4x-2x,
2x-4x, and 2x-2x crosses by using two tetraploids (Solanum tuberosum L.)
and three diploid hybrids of closely related species which can produce 2n
gametes. Forty-three dihaploid plantlets were regenerated from embryoids of
anthers of the three tetraploid hybrids by anther culture in vitro.
Three of them could produce 2n pollen and five of them could produce 2n eggs.
The morphological and agronomic characters of these dihaploids with 2n gametes
were compared with those of their diploid and tetraploid parents. Two dihaploid
clones with 2n gametes and improved agronomic characters were obtained. The
results suggest that ploidy manipulation through sexual polyploidisation and
dihaploid induction by anther culture is an effective method to obtain
dihaploid clones with 2n gametes in combination with good tuber yield and tuber
appearance in potato.
Keywords potato; Solanum tuberosum L.; anther
culture; dihaploid; 2n gametes; agronomic characters
+Corresponding author.
H98013
Received 20 February
1998; accepted 23 November 1999
PDF file of entire paper: medium quality (649K); (scanned from paper original: notes about this process)
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