New Zealand Journal of Crop and Horticultural Science abstracts
Flower pattern stability in genetically modified lisianthus (Eustoma
grandiflorum) under commercial growing conditions
J. MARIE BRADLEY*
S. RAY RAINS
JAN L. MANSON
KEVIN M. DAVIES+
New Zealand Institute for Crop & Food
Research Limited
Private Bag 11 600
Palmerston North, New Zealand
email: daviesk@crop.cri.nz
*Present address: Foundation for Research, Science and
Technology, P. O. Box 12 240, Wellington, New Zealand.
Abstract Lisianthus (Eustoma grandiflorum Grise.) with
novel flower colour patterns have been generated by genetic modification
techniques that alter flavonoid biosynthesis. As described previously, the
level and pattern of pigmentation in purple-flowered lisianthus cultivars was
altered by the introduction of a lisianthus chalcone synthase (CHS) cDNA in an
antisense orientation. As the next step towards commercial development of these
new cultivars, the consistency of altered flower patterns in the progeny and
the performance of these lines under conditions resembling those used by
commercial lisianthus growers in New Zealand have now been assessed. The
introduced antisense CHS transgene had no apparent effect on germination rates
or plant survival. Altered flower patterns observed in the progeny correlated
with the inheritance of the transgenes, as measured by kanamycin resistance and
reduction in CHS expression. At least two of the lines showed sufficient
consistency in flower pattern to warrant continued development. Additionally,
no transfer of the introduced DNA between transgenic and neighbouring
non-transformed lisianthus was observed during this trial.
Keywords chalcone synthase; flavonoids; field trial; flower
colour; genetic modification; lisianthus; Eustoma
H99048
Received 2 December 1999; accepted 14 June 2000
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