New Zealand Journal of Crop and Horticultural Science abstracts
Production of wheat doubled haploids via wide crosses in New Zealand
wheat
A. W. CAMPBELL1,2,*
W. B. GRIFFIN1
D. J. BURRITT3
A. J. CONNER1,2,+
1New Zealand Institute for Crop & Food
Research Limited
Private Bag 4704
Christchurch, New Zealand
2Soil, Plant and Ecological Sciences Division
P. O. Box 84
Lincoln University
Canterbury, New Zealand
3Botany Department
University of Otago
P. O. Box 56
Dunedin, New Zealand
*Present address: Centre for Rural and Environmental Biotechnology,
University of Southern Queensland, Toowoomba, Qld 4350, Australia.
Abstract Doubled haploid (DH) technology allows the
production of homozygous cereal lines in a single generation. The integration
of DH technology into New Zealand wheat (Triticum aestivum) breeding and
genetics programmes has the potential to reduce the breeding time of new
cultivars and improve our understanding of agronomically important genetic
traits. Wheat x maize (Zea mays) crosses are now widely used in the
production of wheat DHs. The aim of this study was to develop a wheat x maize
method of DH production using New Zealand germplasm. A number of New Zealand
wheat cultivars were crossed with different maize genotypes. A successful
method for producing wheat DHs was achieved with all genotypes. Several factors
were identified as being important in the efficiency of wheat DH production.
These included: seasonal constraints, time of embryo excision, method of auxin
application, and culture media composition. Of these factors, seasonal
constraints were the major limitation in the use of the wheat x maize method
for producing DHs in New Zealand.
Keywords wheat; Triticum aestivum; maize; Zea
mays; wide crosses; doubled haploids; breeding; genetics
+Author for correspondence: email: connert@crop.cri.nz
H99045
Received 12 November 1999; accepted 30 June 2000
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