New Zealand Journal of Crop and Horticultural Science abstracts
+Author for correspondence.
H00024Received 11
August 2000; accepted 30 October 2000
Short communication
Survival and recovery of asparagus shoot tips after cryopreservation using
the "droplet" method
G. MIX-WAGNER
Institute of Crop and Grassland Science
Bundesallee 50
38116 Braunschweig, Germany
A. J. CONNER
R. J. CROSS+
New Zealand Institute for Crop & Food
Research Limited
Private Bag 4704
Christchurch, New Zealand
email: crossr@crop.cri.nz
Abstract In vitro cultured shoot tips of asparagus
(
Asparagus officinalis L.) were successfully cryopreserved using the
"droplet" method. Excised shoot tips, pre-treated with liquid cryoprotective
medium, were suspended in small droplets (c. 5 ul) of cryoprotectant on
aluminium foil and then rapidly frozen by direct immersion in liquid nitrogen.
Shoot tips of all eight asparagus genotypes tested were successfully
cryopreserved, and within 7 days had resumed growth into well developed shoots
(5-8 mm long) without intermediate callus formation. High survival and
regrowth was achieved without any cold hardening or pre-growth, and ranged from
58% for clone M120 to 96% for cultivar `Rutgers Beacon', with a mean frequency
of c. 80% across all eight genotypes.
Keywords Asparagus officinalis L.; cryopreservation;
"droplet" method; in vitro culture; genebanks
New Zealand Journal of Crop and Horticultural Science, 2000, Vol. 28:
289-292
0014-0671/00/2804-0289 $7.00 (c) The Royal Society of New Zealand 2000
PDF file of entire paper: medium quality (1117K); (scanned from paper original: notes about this process)
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