New Zealand Journal of Crop and Horticultural Science abstracts
Effect of different treatments on the conversion of `Hayward' kiwifruit
synthetic seeds to whole plants following encapsulation of in
vitro-derived buds
M. ADRIANI
E. PICCIONI
A. STANDARDI+
Department of Arboriculture and Plant Protection
University of Perugia
Borgo XX Giugno, 74
06121, Perugia, Italy
email: asep@unipg.it
Abstract Encapsulated buds excised from
in vitro
proliferated shoots of the `Hayward' kiwifruit (
Actinidia deliciosa (A.
Chev.) C. F. Liang et A. R. Ferguson) can be used for non-embryogenic synthetic
seed production. Three experiments were carried out to evaluate the aptitude of
apical and axillary buds (microcuttings) towards encapsulation and synthetic
seed production, and to find the treatments able to induce conversion of the
synthetic seeds to whole plantlets. `Hayward' proliferating shoots are useful
sources of microcuttings for encapsulation and synseed production, since a
proliferation protocol is already available. Encapsulation, although considered
necessary, depressed microcuttings' vigour and vegetative activity. Cold
treatments provided to the
in vitro proliferating mother shoots boosted
bud vigour and subsequent conversion. Increase of concentration of sucrose in
some steps of the protocol also enhanced conversion, which in some conditions
reached a rate of 57.5%. Potential applications of encapsulation and the
synthetic seed technology in kiwifruit germplasm exchange and commerce are also
discussed.
Keywords kiwifruit; Actinidia deliciosa; artificial
seed; encapsulation; micropropagation; synseed; sodium-calcium alginate
+Corresponding author.
H99028
Received 28 July 1999;
accpeted 24 December 1999
Short communication
PDF file of entire paper: medium quality (1526K); (scanned from paper original: notes about this process)
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