New Zealand Journal of Crop and Horticultural Science abstracts
Distinguishing among Magnolia cultivars using fluorescent Amplified
Fragment Length Polymorphism (AFLP) analysis
ANTHONY D. MITCHELL
ROY A. EDWARDS
Soil, Plant and Ecological Sciences Division
P. O. Box 84
Lincoln University
Lincoln, New Zealand
email (Mitchell): anthony@inet.net.nz
CHRIS M. FRAMPTON
Centre for Computing and Biometrics
P. O. Box 84
Lincoln University
Lincoln, New Zealand
Abstract Amplified Fragment Length Polymorphism (AFLP)
analysis was used to generate informative DNA fingerprints from
Magnolia
denudata,
M. liliiflora, and putative cultivars of their hybrid
cross, i.e.,
M. x
soulangeana,
M.
x soulangeana
`Lennei',
M.
x
soulangeana `Rustica Rubra',
M.
x
soulangeana `Alba',
M.
x
soulangeana
`Ruby', and
M. x
soulangeana `San Jose'. A single specimen of
unknown origin and representatives of the closely related
M.
campbellii and
M.
stellata were also included in the
analysis. We show how this method may be used for distinguishing among
cultivars.
Keywords Amplified Fragment Length Polymorphism (AFLP);
cultivar; DNA fingerprinting; hybrid; Magnolia
H00051
Received 19 December 2000; accepted 8 May 2001
New Zealand Journal of Crop and Horticultural Science, 2001, Vol. 29:
77-83
0014-0671/01/2902-0077 $7.00 (c) The Royal Society of New Zealand 2001
PDF file of entire paper: medium quality (529K); (scanned from paper original: notes about this process)
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