New Zealand Journal of Crop and Horticultural Science abstracts
Short communication:
Effect of cold treatment on precocious germination in somatic
embryogenesis of wheat (Triticum aestivum)
K. H. KIAROSTAMI
H. EBRAHIMZADEH
Department of Biology
Faculty of Science
Tehran University
Tehran, Iran
email: ebizadeh@khayam.ut.ac.ir
Abstract Immature embryos are the best explants for induction
of somatic embryogenesis. However, precocious germination of these explants is
a difficult problem facing this technology in certain wheat (
Triticum
aestivum L.) cultivars. To overcome this problem, spikes were harvested at
10-14 days post
-anthesis, sterilised, and stored under two different
conditions (4[[ordmasculine]]C for 4, 7, and 10 days and 8deg.C for 4, 7, and
10 days). Significant differences
occurred in the germination response
between treated and control samples, although no significant differences were
observed in embryogenic tissue induction between the samples. A lower rate
of germination
was observed in the spikes
that were
maintained at 4deg.C for 7 and 4 days.
Keywords somatic embrygenesis; wheat; precocious germination;
cold treatment
H00016
Received 20 June 2000; accepted 11 December 2000
New Zealand Journal of Crop and Horticultural Science, 2001, Vol. 29:
209-212
0014-0671/01/2903-0209 $7.00 (c) The Royal Society of New Zealand
2001
PDF file of entire paper: medium quality (541K); (scanned from paper original: notes about this process)
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