New Zealand Journal of Crop and Horticultural Science abstracts
In vitro propagation of Leptospermum hybrids
J. F. SEELYE
B. L. HOFMANN
G. K. BURGE
E. R. MORGAN
New Zealand Institute for Crop & Food
Research Limited
Private Bag 11 600
Palmerston North, New Zealand
Abstract Tissue culture protocols were developed for superior
ornamental
Leptospermum hybrids. Benzylaminopurine (BAP) was the most
effective cytokinin for the proliferation of shoots. On modified Murashige
& Skoog (MS) media with low BAP concentrations (0.05-0.1 mg
litre
-1), an average of up to nine shoots developed from single
nodes, although there were significant differences between the clones. Mean
shoot length was relatively short for all clones growing in the presence of
BAP. Adding gibberellic acid (GA3) to the proliferation medium increased shoot
length only at the highest rate assessed (10 mg litre
-1), but this
resulted in shoots that were spindly and chlorotic and reduced in number. A
reduction in the culture room light level slightly reduced shoot length. Shoots
developed roots, either on a growth regulator-free medium, or on a medium with
low concentrations of indoleacetic acid (IAA) or indolebutyric acid (IBA).
Rooted plantlets were successfully transferred to the greenhouse environment.
Keywords Leptospermum; tissue culture; proliferation;
rooting
H00048
Received 1 December 2000; accepted 14 September 2001
New Zealand Journal of Crop and Horticultural Science, 2001, Vol. 29:
233-237
0014-0671/01/2904-0233 $7.00 (c) The Royal Society of New Zealand 2001
PDF file of entire paper: medium quality (384K); (scanned from paper original: notes about this process)
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