New Zealand Journal of Crop and Horticultural Science abstracts
Hordeum vulgare x H. bulbosum tetraploid hybrid
provides useful agronomic introgression lines for breeders
LIANGTAO ZHANG
School of Biological Sciences
The University of Auckland
Private Bag 92 019
Auckland, New Zealand
R. A. PICKERING
New Zealand Institute for Crop & Food
Research Limited
Private Bag 4704
Christchurch, New Zealand
B. G. MURRAY
School of Biological Sciences
The University of Auckland
Private Bag 92 019
Auckland, New Zealand
Abstract A sterile hybrid between barley and
Hordeum
bulbosum L. that showed regular chromosome pairing at meiotic metaphase was
treated with colchicine to induce polyploidy to restore fertility. Selfed
diploid progenies of this hybrid were screened for resistance to leaf rust and
powdery mildew and the presence of other
H. bulbosum-like characters. A
combination of fluorescent
in situ hybridisation (FISH) and genomic
in situ hybridisation (GISH) was used to identify and characterise
introgressed chromosome segments of
H. bulbosum chromatin and to locate
them to specific barley chromosomes. The size of introgression segments varied
considerably between different plants. This material provides valuable new
germplasm for breeding disease resistant barley.
Keywords barley; disease resistance; FISH; GISH; Hordeum
bulbosum; interspecific hybridisation; introgression
PDF file of entire paper: medium quality (910K); (scanned from paper original: notes about this process)
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