New Zealand Journal of Crop and Horticultural Science abstracts
Genetic diversity in New Zealand populations of Sclerotium cepivorum
J. L. Tyson1
H. J. Ridgway2
R. A. Fullerton1
A. Stewart2
1
The Horticulture and Food Research
Institute of New Zealand Ltd
Mt Albert Research Centre
Private Bag 92 169
Auckland, New Zealand
email: jtyson@hortresearch.co.nz
2
Soil, Plant and Ecological Sciences Division
P.O. Box 84
Lincoln University
Canterbury, New Zealand
Abstract
Mycelial interactions were examined between 231 New Zealand isolates of Sclerotium cepivorum
Berk., as well as 25 isolates from a further eight countries. Within the group of New Zealand isolates six mycelial compatibility groups (MCGs) were identified and named using reference strains obtained from the University of Guelph, Canada. Two new groups, presently only known from New Zealand, were identified. Heterogeneity was found both within and between fields in all regions. A subset of 51 S. cepivorum
isolates was further investigated for genetic diversity using universally primed PCR (UP-PCR) and random amplified polymorphic DNA (RAPD) analyses. When results were analysed separately, each primer distinguished one to three genetic groups. However, when the results from two primers (L15 and OPAX15) were combined, six genetic groups were revealed. The results, as shown by MCGs and molecular methods, demonstrate the presence of genetic diversity in New Zealand field populations of S. cepivorum
.
Keywords
Sclerotium cepivorum; Allium
white rot; mycelial compatibility groups; genetic diversity; UP-PCR; RAPD; New Zealand
H01019 Received 16 May 2001; accepted 13 December 2001
New Zealand Journal of Crop and Horticultural Science, 2002, Vol. 30
: 37–48
0014–0671/02/3001–0037 $7.00 © The Royal Society of New Zealand 2002
PDF file of entire paper: Print-quality (4102K)
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