New Zealand Journal of Crop and Horticultural Science abstracts
RAPD-PCR and UP-PCR techniques distinguish a Pithomyces chartarum
isolate with biocontrol capabilities against Botrytis cinerea on grape
(Vitis vinifera)
S. L. Dodd
A. Stewart†
Soil Plant and Ecological Sciences Division
P.O. Box 84
Lincoln University
Canterbury, New Zealand
email: stewarta@lincoln.ac.nz
†Corresponding author.
Abstract Previous work identified an Ulocladium
atrum (LU850) isolate with potential as biocontrol against Botrytis on
grape (Vitis vinifera). The aim of this study was to find a molecular
marker that would distinguish the biocontrol isolate from other isolates
of the same species and closely related genera to enable subsequent monitoring
of its survival, establishment, and spread in the field. DNA of 53 fungal
isolates representing the three genera Ulocladium, Alternaria,
and Pithomyces were screened with 10 universally primed polymerase
chain reaction (UP-PCR) primers and 28 random amplified polymorphic DNA (RAPD-PCR)
primers to screen for bands unique to the biocontrol isolate. Three RAPD-PCR
primers produced a single band unique to isolate LU850. However, resultant
band patterns and subsequent more detailed morphological studies revealed
the biocontrol isolate to be Pithomyces chartarum and not U. atrum.
Since some isolates of P. chartarum produce the toxin sporidesmin,
which is responsible for facial eczema in cattle and sheep, the toxin producing
status of isolate LU850 will need to be determined before it can be considered
for further development as a biological control agent.
Keywords molecular marker; Ulocladium; Pithomyces
chartarum
H02051 Received 29 July 2002; accepted 15 January 2003; published 26 March
2003
New Zealand Journal of Crop and Horticultural Science, 2003, Vol. 31:
55-64
0014-0671/03/3101-0055 $7.00 © The Royal Society of New Zealand
2003
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