New Zealand Journal of Crop and Horticultural Science abstracts
Production of tetraploid Gentiana triflora var. japonica
‘Royal Blue’ plants
E. R. Morgan
B. L. Hofmann
New Zealand Institute for Crop & Food
Research Limited
Private Bag 11 600
Palmerston North, New Zealand
email: morgane@crop.cri.nz
J. E. Grant
New Zealand Institute for Crop & Food
Research Limited
Private Bag 4704
Christchurch, New Zealand
Abstract The aim of this work was to test a protocol
for producing tetraploid plants of Gentiana. Tetraploid plants were
produced by growing in vitro cultures of Gentiana triflora
var. japonica ‘Royal Blue’ in the presence of oryzalin for 4 weeks.
A population of plants was regenerated from these in vitro cultures
and transferred to the greenhouse. The treated plants were screened for higher
ploidy level on the basis of leaf thickness and further confirmation of polyploidy
was provided by measuring guard cell lengths. The tetraploid nature of one
plant with larger than normal guard cells was confirmed by flow cytometry
and chromosome counts. The mean nuclear DNA contents of 2C and 4C nuclei
were 9.26 pg and 17.77 pg DNA, respectively. Mitotic chromosome counts from
diploid and tetraploid plants were 2n=26 and 2n=52 respectively.
Keywords chromosome number; flow cytometry; gentian;
oryzalin; tetraploids
H02096 Received 9 December 2002; accepted 10 February 2003; published
26 March 2003
New Zealand Journal of Crop and Horticultural Science, 2003, Vol. 31:
65-68
0014-0671/03/3101-0065 $7.00 © The Royal Society of New Zealand
2003
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