New Zealand Journal of Crop and Horticultural Science abstracts
Using ELISA and PCR to test the potential for spread of plum pox virus
by seeds of different stone fruit cultivars
T. Thomidis
I. Karajiannis
National Agricultural Research Foundation
Pomology Institute
Naoussa, P.C. 59200
Greece
email: tomgr@otenet.gr
Abstract The enzyme-linked immunosorbent assay (ELISA)
and the polymerase chain reaction (PCR) were used to test the potential for
spread of plum pox virus (PPV) by seeds of the apricot (Prunus armeniaca)
varieties ‘Tyrinthos’ and ‘Bebecou’, and the stone fruit rootstocks ‘GF305’
(Prunus persica) and ‘Myrobalan’ (Prunus cerasifera). Seeds
originating from infected ‘Tyrinthos’, ‘Bebecou’, ‘GF305’, and ‘Myrobalan’
trees were stratified in metal boxes containing sand in a screenhouse. Leaves
were collected from each of the seedlings and tested for PPV. No seedling
was found infected with this pathogen. Our results suggest that at least
in the cultivars tested, PPV is not seed transmitted.
Keywords seeds; spread; stone fruit; virus
H02041 Received 30 May 2002; accepted 25 September 2002; published 26
March 2003
New Zealand Journal of Crop and Horticultural Science, 2003, Vol. 31: 69-72
0014-0671/03/3101-0069 $7.00 © The Royal Society of New Zealand
2003
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