New Zealand Journal of Crop and Horticultural Science abstracts
Organogenesis potential for kiwifruit (Actinidia deliciosa) cuttings
and pedicel treated with Agromil-Plus®
J. G. Cruz-Castillo
Universidad Autónoma Chapingo
Centro Regional Universitario Oriente
Apartado Postal 49
Huatusco, Veracruz 94100
Mexico
email: jcruzcastillo@yahoo.com
D. J. Woolley
Institute of Natural Resources
Massey University
Private Bag 11 222, Palmerston North
New Zealand
Abstract A preliminary evaluation was made of the organogenesis
capability of kiwifruit (Actinidia deliciosa) pedicels in vivo
and orchard cuttings in vitro, using Agromil-Plus® (Agroenzymas
Laboratories, Tlanepantla, Mexico) which is a mixture of CPPU (N-(2-chloro-4-pyridy)-N’-phenylurea)
with natural bioregulators. ‘Hayward’ kiwifruit (A. deliciosa var.
deliciosa) cuttings were submerged in a solution with a final concentration
of 10 µl/litre CPPU plus natural bioregulators. Leafy shoot formation
from callus was observed in 10% and 30% of the cuttings treated with Agromil-Plus®
on the distal and proximal ends, respectively. For pedicel treatments, after
the fruitlet was removed, the pedicel was inserted in a vial filled with
Agromil-Plus®. Leafy shoot formation from callus was recorded in 20%
of the treated pedicels. Some cuttings from field-grown plants and pedicels
were capable of producing shoots when they were treated with CPPU plus natural
plant growth regulators.
Keywords callus production; cytokinins; diphenylurea;
indirect organogenesis
H02039 Received 29 May 2002; accepted 4 April 2003 ; published 12 June
2003
New Zealand Journal of Crop and Horticultural Science, 2003, Vol. 31:
193-196
0014-0671/03/3102-0193 $7.00 © The Royal Society of New Zealand
2003
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