New Zealand Journal of Crop and Horticultural Science abstracts
Use of in ovulo embryo culture to produce interspecific hybrids
between Gentiana triflora and Gentiana lutea
E. R. Morgan
New Zealand Institute for Crop and Food
Research Limited
Private Bag 11 600
Palmerston North, New Zealand
email: morgane@crop.cri.nz
Abstract Gentiana is increasingly being exploited as
a cut flower crop but its colour range in commercial varieties is limited
despite a wide range of colours being present in the genus. The aim of this
work was to produce interspecific hybrids between G. triflora and
G. lutea with the ultimate aim being to produce yellow-flowered cultivars.
Six to 8-day-old embryos were rescued from the G. triflora parent
and cultured within their ovules on modified B5 medium. Following embryo
germination, plantlets were transferred to modified Murashige & Skoog
(MS) medium for further growth and proliferation. Shoots were then transferred
to growth-regulator free, modified MS medium for root initiation before transfer
to the greenhouse. However, plants died within a few months of transfer to
the greenhouse. The hybrid nature of the embryo-derived plants was indicated
first by flow cytometry, then confirmed by chromosome counts and examination
of morphological features. Mitotic chromosome counts of one of the G.
triflora x G. lutea hybrid plants gave a chromosome number 2n
= 33.
Keywords chromosome number; embryo rescue; flow cytometry;
wide cross
H04058; Received 24 June 2004; accepted 15 September 2004 Online publication
date 11 November 2004
New Zealand Journal of Crop and Horticultural Science, 2004, Vol. 32:
343–347
0014–0671/04/3204–0343 $7.00 © The Royal Society of New Zealand
2004
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