New Zealand Journal of Crop and Horticultural Science abstracts
Population variability in wild turnip (Brassica rapa var.oleifera)
for interspecific hybridisation with herbicide-resistant rape Brassica
napus) pollen
T. E. Jenkins1
C. M. Frampton2,*
A. J. Conner1,3
1National Centre for Advanced Bio-Protection Technologies
P.O. Box 84, Lincoln University
Canterbury, New Zealand
2Applied Management and Computing Division
P.O. Box 84, Lincoln University
Canterbury, New Zealand
3New Zealand Institute for Crop and Food
Research Limited
Private Bag 4704
Christchurch, New Zealand
email:connert@crop.cri.nz
*Present address: Christchurch School of Medicine and Health Sciences,
P.O. Box 4345, Christchurch, New Zealand.
Abstract As a basis for risk assessment for the release
of transgenic rape (Brassica napus) plants, this study investigates
the potential for hybrid formation between a non-transgenic herbicide-resistant
rape (AACC; 2n = 4x = 38) as the pollen parent and six New Zealand populations
of wild turnip (Brassica rapa var.oleifera) (AA; 2n = 2x = 20)
as the maternal parent. Following hand pollination, 41% of pollinated stigma
developed into siliques with seeds. The frequency of successful pollination
varied significantly between wild turnip populations and was higher on flowers
from lower racemes than upper racemes. The wild turnip populations also differed
in the number of seeds per silique. In all populations, fewer seeds developed
in siliques from the upper raceme compared with the lower raceme. Over 99%
of successfully germinated progeny were chlorsulfuron-resistant, with the
seed germinating as chlorsulfuron-sensitive seedlings (0.7%) presumed to be
matromorphic seed. Flow cytometry established that the chlorsulfuron-resistant
seedlings were triploids, thereby confirming their hybrid status. The frequency
of dormant seeds did not differ between wild turnip populations, although
the upper racemes produced 6-fold more dormant seed than the lower racemes.
Keywords wild turnip; rape; Brassica napus;Brassica
rapa var. oleifera; interspecific hybrids; hybridisation; triploids;
flow cytometry
H03098; Online publication date 8 March 2005 Received 23 October 2003;
accepted 22 November 2004
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